1. Parte do relatório final de iniciação cientifica do primeiro autor, financiado pelo PROBIC/FAPEMIG e Edital Recém-contratados, PRPQ/UFMG.
   
2. Alunos do curso de graduação em Zootecnia UFMG/NCA, Montes Claros-MG. e-mail: tatiany_xavier@yahoo.com.br
   
3. Prof. Adj. Dept. Agronomia UFMG/Núcleo de Ciências Agrárias. Montes Claros-MG.-Orientadores

Resumo: Objetivou-se avaliar a atividade antimicrobiana em cepas de Staphylococcus coagulase positivo e negativo, Streptococcus esculina positivo e negativo, e Manheymia haemolytica isoladas de leite mastítico de ovelhas, frente á extratos de barbatimão. Utilizaram-se técnicas de macrodiluição em tubos e difusão em discos com concentrações de 100, 200, 300 e 400ug/ml do extrato. Os resultados demonstraram que nas 5 amostras de Staphylococcus coagulase positivo, 2(40%) observou-se CIM em 400ug/ml do extrato; 1(20%) em 300ug/ml e 1(20%) em 200ug/ml. No teste de difusão em discos, teve-se média de halos de inibição 14,2mm na concentração de 400ug/ml; 12,6mm na 300ug/ml; 11mm na 200ug/ml e 8,4mm na 100ug/ml. Nas 5 amostras de Staphylococcus coagulse negativo, 100% tiveram CIM em 400ug/ml do extrato; os halos de inibição do teste de difusão em discos, tiveram média de 12,6mm em 400ug/ml, 12,0mm para a de 300 e 200ug/ml e 10,4mm para a de 100ug/ml. Das 5 amostras de Streptococcus esculina positivo, em 1(20%) obteve-se a CIM, que foi de 400ug/ml do extrato. A média dos halos de inibição foram de 9mm para 400ug/ml, 3,8mm para 300ug/ml, 1,8mm para 200ug/ml e 1,2 para a de 100ug/ml. Nas 5 amostras de Streptococcus esculina negativo, 3(60%) obteve-se a CIM, que foi de 400ug/ml; os halos de inibição tiveram média de 4,6mm para 400ug/ml, 3,2mm para 300ug/ml, e 0,8mm para 200ug/ml. Nas 5 amostras de Manheymia haemolytica a média de halos de inibição na concentração de 400ug/ml foi de 1,4mm.

Palavras-chave: Concentração inibitória mínima, fitoterapia, plantas medicinais, resistência antimicrobiana, tratamentos alternativos

Assessment of activity in vitro antimicrobial extract of the stryphnodendron adstringens front of a microorganisms isolatyed from mastitis in ewes, in the Minas Gerais

Abstract: It was had for objective to evaluate the antimicrobial activity of strainsof positive Staphylococcus coagulase, negative Staphylococcus coagulase, positive esculin Streptococcus, isolated negative esculin Streptococcus and haemolytica Manheymia of mastitic milk of sheep of the race Saint Ines, front the hidroalcoholic extracts of Stryphnodendron adstringens. The techniques of macrodilution in pipes and diffusion in records with concentrations of 100ug/ml of extract had been used; 200ug/ml; 300ug/ml and 400ug/ml, front the standardized bacterial inocula in 0,5 of the scale of Mac Farland. The results of the use of the extract of the rind of the barbatimão in the 5 samples of positive Staphylococcus coagulase, had demonstrated that in 2 (40%) CIM in 400ug/ml of the extract were observed; 1 (20%) in 300ug/ml and 1 (20%) in 200ug/ml. In the test of diffusion in records, 14,2mm for the concentration was observed average of inhibition halos 400ug/ml of the extract; 12,6mm for 300ug/ml; 11mm for 200ug/ml and 8,4mm for 100ug/ml. For the 5 samples of negative Staphylococcus coagulse, all (100%) had had CIM in 400ug/ml of the extract; e the halos of inhibition of the test of diffusion in records, had had average of 12,6mm for the concentration of 400ug/ml of the extract, 12,0 mm for the one of 300 and 200ug/ml and 10,4mm for the one of 100ug/ml. Of the 5 samples of positive esculin Streptococcus, only in 1 (20%), it was gotten CIM, which was in 400ug/ml of the extract. The average of halos of inhibition for these samples had been of 9mm for 400ug/ml of the extract, 3,8mm for 300ug/ml of the extract, 1,8mm for the concentration of 200ug/ml and 1,2 for the one of 100ug/ml. In the 5 samples of negative esculin Streptococcus, in 3 (60%) it was only gotten CIM, that it was of 400ug/ml; already the inhibition halos had had average of 4,6mm for the concentration of 400ug/ml, 3,2mm for the one of 300ug/ml, 0,8mm for the one of 200ug/ml and 0mm for the one of 100ug/ml. In the 5 samples of haemolytica Manheymia, it was not gotten CIM with the established concentrations of the extract; e only perceived a halo average of inhibition in the concentration of 400ug/ml of the extract that was of 1,4mm. For all the samples that if tested with the extract of the barbatimão, the CBM and the anti-septic activity had been found, by means of the concentrations that had been used of the extract.

Keywords: Minimum inhibitory concentration, phytotherapy, medicinal plants, antimicrobial resistance, alternative treatments

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